S-IgA
分泌型免疫球蛋白A(secretory immunoglobulin A,SIgA)是20世纪60年代初在外分泌液中发现的一种IgA抗体,主要存在于乳汁、胃肠液、呼吸道分泌液等外分泌液中。SIgA分子是由2个IgA单体(每个单体含2条轻链和2条重链)、1条J链和1条分泌片(secretory component,SC,为多聚免疫球蛋白受体的胞外裂解片段)构成的异源十聚体,为了与血清IgA单体相区别而被命名为SIgA。SIgA是外分泌液中存在的一种主要抗体,是呼吸道、消化道、泌尿生殖道等抵御病原体及有害物质的第一道免疫防线,是机体黏膜免疫最重要的抗体。
可检测样本中小鼠的sIgA,且与其类似物无明显交叉反应。
由于实验操作条件的不同( 如操作者、移液技术、洗板技术和稳定条件等),标准曲线的OD值会有所差异。以下数据和曲线仅供参考,实验者需根据自己的实验建立标准曲线。
板内变异系数小于10%,板间变异系数小于10%
在选取的健康小鼠血清、血浆、细胞培养上清中加入3个不同浓度水平的小鼠sIgA,计算回收率
分别在选取的4份健康小鼠血清、血浆、细胞培养上清中加入高浓度小鼠sIgA,在标准曲线动力学范围内进行稀释,评估线性。
注:ELISA试剂盒检测范围等数据,因检测样本 的不同而调整,实际数据以随货说明书为准。
Effect of the β-glucan from Lentinus edodes on colitis-associated colorectal cancer and gut microbiota
Rank: IF 10.7
Journal: Carbohydrate Polymers
Year: 2023
DOI: 10.1016/j.carbpol.2023.121069
Self-adjuvant multiepitope nanovaccine based on ferritin induced long-lasting and effective mucosal immunity against H3N2 and H1N1 viruses in mice
Rank: IF 7.7
Journal: International Journal of Biological Macromolecules
Year: 2024
DOI: 10.1016/j.ijbiomac.2024.129259
Polysaccharide from Areca catechu L. inflorescence enhances the intestinal mucosal immunity to maintain immune homeostasis
DOI: 10.1016/j.ijbiomac.2024.134900
Lactobacillus casei ATCC 393 combined with vasoactive intestinal peptidealleviates dextran sodium sulfate-induced ulcerative colitis in C57BL/6mice via NF-κB and Nrf2 signaling pathways
Rank: IF 6.9
Journal: Biomedicine & Pharmacotherapy
DOI: 10.1016/j.biopha.2023.115033
Beneficial effects of Dendrobium officinale National Herbal Drink on metabolic immune crosstalk via regulate SCFAs-Th17/Treg
Rank: IF 6.7
Journal: Phytomedicine
DOI: 10.1016/j.phymed.2024.155816
Dietary supplementation with biogenic selenium nanoparticles alleviate oxidative stress-induced intestinal barrier dysfunction
Rank: IF 6.3
Journal: npj Science of Food
Year: 2022
DOI: 10.1038/s41538-022-00145-3
Prophylactic supplementation with selenium nanoparticles protects against foodborne toxin zearalenone-induced intestinal barrier dysfunction
Rank: IF 6.2
Journal: Ecotoxicology and Environmental Safety
DOI: 10.1016/j.ecoenv.2024.116914
Replacing dietary sodium selenite with biogenic selenium nanoparticles improves the growth performance and gut health of early-weaned piglets
Rank: IF 6.1
Journal: Animal Nutrition
DOI: 10.1016/j.aninu.2023.08.003
Fucoidan-Mediated Covalent Modification Mitigates Allergenicity of Shrimp (Penaeus vannamei) in Mice via Enhanced Intestinal Barrier Function and Antigen Presentation Suppression
Rank: IF 5.7
Journal: Journal of Agricultural and Food Chemistry
DOI: 10.1021/acs.jafc.4c09800
Polygonatum sibiricum Saponin Prevents Immune Dysfunction and Strengthens Intestinal Mucosal Barrier Function in Cyclophosphamide-Induced Immunosuppressed BALB/c Mice
Rank: IF 4.7
Journal: Foods
DOI: 10.3390/foods13060934