NOS2A;NOS2; HEP-NOS; I-NOS; Hepatocytes Oxide Synthase; Peptidyl-cysteine S-nitrosylase NOS2
诱导型NO合酶(iNOS)是由氨基酸l-arginine生成一氧化氮(NO)的三种关键酶之一。INOS衍生的NO在许多生理(如血压调节、伤口修复和宿主防御机制)和病理生理(炎症、感染、肿瘤性疾病、肝硬化、糖尿病)条件下发挥着重要作用。然而,INOS在肿瘤发展过程中的作用是非常复杂的,如蜕变、血管生成和转移,是由INOS调节的。另一方面,来自巨噬细胞的NO对肿瘤细胞有潜在的细胞毒性/细胞抑制作用。因此,对INOS活动的治疗干预具有相当大的意义,特别是在肿瘤中,转移活动、宿主防御机制和分化水平与INOS的表达相关。
可检测样本中小鼠的iNOS,且与其类似物无明显交叉反应。
由于实验操作条件的不同( 如操作者、移液技术、洗板技术和稳定条件等),标准曲线的OD值会有所差异。以下数据和曲线仅供参考,实验者需根据自己的实验建立标准曲线。
注意:本图仅供参考,应以同次试验标准品所绘标准曲线计算标本含量。
板内变异系数小于10%,板间变异系数小于10%
在选取的健康小鼠组织匀浆、细胞培养上清中加入3个不同浓度水平的小鼠iNOS,计算回收率
分别在选取的4份健康小鼠组织匀浆、细胞培养上清中加入高浓度小鼠iNOS,在标准曲线动力学范围内进行稀释,评估线性。
注:ELISA试剂盒检测范围等数据,因检测样本 的不同而调整,实际数据以随货说明书为准。
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