Interleukin 12
白细胞介素12(IL-12)是一种白细胞介素,由树突状细胞、巨噬细胞、中性粒细胞和B淋巴细胞(NC-37)在抗原刺激下自然产生。IL-12属于白细胞介素-12家族,在自然杀伤细胞和T淋巴细胞的活动中起重要作用。IL-12还具有抗血管生成活性,这意味着它可以阻止新血管的形成。IL-12的是由二硫键相连异源二聚体,70 kDa (p70)的 异二聚体糖蛋白由40 kDa (p40)和35 kDa (p35)两个亚基组成。
可检测样本中小鼠的IL-12,且与其类似物无明显交叉反应。
由于实验操作条件的不同( 如操作者、移液技术、洗板技术和稳定条件等),标准曲线的OD值会有所差异。以下数据和曲线仅供参考,实验者需根据自己的实验建立标准曲线。
注意:本图仅供参考,应以同次试验标准品所绘标准曲线计算标本含量。
板内变异系数小于10%,板间变异系数小于10%
在选取的健康小鼠血清、血浆、细胞培养上清中加入3个不同浓度水平的小鼠IL-12,计算回收率
分别在选取的4份健康小鼠血清、血浆、细胞培养上清中加入高浓度小鼠IL-12,在标准曲线动力学范围内进行稀释,评估线性。
注:ELISA试剂盒检测范围等数据,因检测样本 的不同而调整,实际数据以随货说明书为准。
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