E2
雌二醇(Estradiol,E2),亦称“动情素”、“求偶素”。化学式 C18H24O2, 分子量为 272.38,有α,β两种类型,雌激素的一种。含量最多,活性也最强。由卵巢内卵泡的颗粒细胞分泌,其代谢物是雌酮及雌三醇。含 18 个碳原子。其靶器官为子宫、阴道、输卵管和垂体。可作为一种为经皮肤吸收的 雌激素治疗剂。雌激素能促使细胞合成 DNA、RNA 和相应组织内各种不同的蛋白质。
可检测样本中小鼠的E2,且与其类似物无明显交叉反应。
由于实验操作条件的不同( 如操作者、移液技术、洗板技术和稳定条件等),标准曲线的OD值会有所差异。以下数据和曲线仅供参考,实验者需根据自己的实验建立标准曲线。
板内变异系数小于10%,板间变异系数小于10%
在选取的健康小鼠血清、血浆和细胞培养上清中加入3个不同浓度水平的小鼠E2,计算回收率
分别在选取的4份健康小鼠血清、血浆和细胞培养上清中加入高浓度小鼠E2,在标准曲线动力学范围内进行稀释,评估线性。
注:ELISA试剂盒检测范围等数据,因检测样本 的不同而调整,实际数据以随货说明书为准。
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Rank: IF 7.5
Journal: Biomedicine & Pharmacotherapy
Year: 2024
DOI: 10.1016/j.biopha.2024.116361
Single-nucleus RNA Sequencing reveals the mechanism of cigarette smoke exposure on diminished ovarian reserve in mice
Rank: IF 6.1
Journal: Ecotoxicology and Environmental Safety
Year: 2022
DOI: 10.1016/j.ecoenv.2022.114093
Di-(2-ethylhexyl) phthalate exposure induces ferroptosis by regulating the Nrf2-mediated signaling pathway in mouse ovaries
DOI: 10.1016/j.ecoenv.2024.117104
Danggui-Shaoyao-San improves cognitive impairment through inhibiting O-GlcNAc-modification of estrogen α receptor in female db/db mice.
Rank: IF 5.4
Journal: Journal of Ethnopharmacology
Year: 2021
DOI: 10.1016/j.jep.2021.114562
Exploring the efficacy and mechanism of Bailing capsule to improve polycystic ovary syndrome in mice based on intestinal-derived LPS-TLR4 pathway
DOI: 10.1016/j.jep.2024.118274
DNA Methylation Mediates the Transcription of STAT4 to Regulate KISS1 During Follicular Development
Rank: IF 5.2
Journal: Cells
Year: 2025
DOI: 10.3390/cells14070523
Deer Blood Hydrolysate Protects against D-Galactose-Induced Premature Ovarian Failure in Mice by Inhibiting Oxidative Stress and Apoptosis
Rank: IF 5
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DOI: 10.3390/nu16203473
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Rank: IF 4.9
Journal: International Journal of Molecular Sciences
Year: 2023
DOI: 10.3390/ijms242115666
Study on pharmacokinetic and tissue distribution of hyperin, astragalin, kaempferol-3-O-β-D-glucuronide from rats with multiple administrations of Semen Cuscutae processed with salt solution with effect of treating recurrent spontaneous abortion
Rank: IF 4.8
Journal: Frontiers in Pharmacology
DOI: 10.3389/fphar.2024.1440810
Behavior, hormone, and gut microbiota change by YYNS intervention in an OVX mouse model
Journal: Frontiers in Cellular and Infection Microbiology
DOI: 10.3389/fcimb.2024.1445741