Transforming growth factor beta
转化生长因子β(TGF-β)是一种多功能的细胞因子,属于转化生长因子超家族,包括三种不同的哺乳动物异构体(TGF-β 1至3,HGNC符号TGFB1、TGFB2、TGFB3)和许多其他信号蛋白。TGFB蛋白由所有白血球系产生。被激活的TGF-β与其他因子复合形成丝氨酸/苏氨酸激酶复合物,与TGF-β受体结合。TGF-β受体由1型和2型受体亚单位组成。
可检测样本中小鼠的TGF-β,且与其类似物无明显交叉反应。
由于实验操作条件的不同( 如操作者、移液技术、洗板技术和稳定条件等),标准曲线的OD值会有所差异。以下数据和曲线仅供参考,实验者需根据自己的实验建立标准曲线。
注意:本图仅供参考,应以同次试验标准品所绘标准曲线计算标本含量。
板内变异系数小于10%,板间变异系数小于10%
在选取的健康小鼠血清、血浆、细胞培养上清中加入3个不同浓度水平的小鼠TGF-β,计算回收率
分别在选取的4份健康小鼠血清、血浆、细胞培养上清中加入高浓度小鼠TGF-β,在标准曲线动力学范围内进行稀释,评估线性。
注:ELISA试剂盒检测范围等数据,因检测样本 的不同而调整,实际数据以随货说明书为准。
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